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What is a good value for BLAST?
What is a good value for BLAST?
CryptoTitanGuard
Sat Oct 19 2024
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7 answers
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I'm trying to figure out what would be considered a good value when using BLAST. I understand it's a tool for sequence alignment, but I want to know what constitutes a significant or reliable result in terms of the value it generates.
7 answers
Chloe_carter_model
Mon Oct 21 2024
The criteria for evaluating the quality of an E-value obtained from a BLAST search are crucial in determining the significance of a sequence alignment.
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EmilyJohnson
Mon Oct 21 2024
In the realm of bioinformatics, a good E-value threshold is considered essential for filtering out insignificant matches and focusing on biologically relevant results.
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isabella_bailey_economist
Mon Oct 21 2024
Typically, a threshold of e−5, which translates to 1 in 100,000 chances of obtaining a match by random chance, is deemed satisfactory for many applications.
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Bianca
Mon Oct 21 2024
This threshold ensures that only alignments with a high degree of statistical significance are considered, thereby minimizing false positives.
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PulseWind
Sun Oct 20 2024
The E-value calculation takes into account both the length of the alignment and the overall frequency of the matching sequences in the database being searched.
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